http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104359734-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
| filingDate | 2014-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104359734-B |
| titleOfInvention | Production method for fluorescent microscopic slices of ovule of pollinated azalea |
| abstract | The invention provides a production method for fluorescent microscopic slices of ovule of pollinated azalea. The production method comprises the following steps: sampling pistil of pollinated azalea; and fixing, softening, dyeing, stripping ovary walls and tabletting. In the steps of sampling and fixing, pollinated azalea flowers are picked, flower petals are stripped, only pistil parts comprising stigmas and ovaries are remained and are put into an FAA fixing solution to be fixed, and after 24 hours, the pistil parts are converted into a 70% ethanol solution to be stored; in the softening process, the fixed materials are put into a drying oven to be heated and softened; in the dyeing process, the softened materials are dyed with a 0.1% water-soluble aniline blue dyeing solution without light for one night; in the ovary wall stripping process, the dyed materials are subjected to ovary wall stripping treatment; and in the tabletting process, tabletting is performed on the stripped materials to obtain the slices. According to the method, the slices of ovule of pollinated azalea, which are clearly dyed and have complete tissue structure, can be obtained; and comprehensive fertilization information can be observed. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106950088-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106950088-B |
| priorityDate | 2014-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.