http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104357918-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
| filingDate | 2014-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104357918-B |
| titleOfInvention | The construction method in plasma DNA library |
| abstract | The invention provides one " construction method of a small amount of DNA library ", first plasma DNA or fragmentation DNA are carried out end reparation, and 5 ' ends is carried out phosphorylation modification simultaneously, DNA after being repaired；Afterwards directly and manual splice carries out blunt end cloning, purer junction band is obtained after purified jaggy containing linker DNA；Then PCR polymerase was used to carry out the repairing of breach before PCR expands, complete containing linker DNA after being repaired；Carry out pcr amplification reaction again, after product purification, obtain final high-throughput sequencing library.This method is not only simple, efficiently, decrease purification step, reduce the loss of DNA in library construction process, and use specific joint and the connected mode of flat end, efficiently solve and minim DNA library construction process easily occurs joint from the problem connected；Meanwhile, breach is repaired before amplification, it is ensured that before amplification, have enough complete templates to carry out amplified reaction. |
| priorityDate | 2014-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 84.