http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104357440-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2014-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104357440-B |
| titleOfInvention | Target the Talen recognition sequences and its mRNA preparation methods of zebra fish Forkhead box n1 genes for a pair |
| abstract | The invention discloses the Talen recognition sequences and the preparation method of mRNA for targeting knock out zebra fish Forkhead box n1 genes of a pair of targeting zebra fish Forkhead box n1 genes.Respectively as shown in sequence table SEQ ID NO.1 and SEQ ID NO.2, the preparation method of the mRNA comprises the following steps the Talen recognition sequences pair for a) assembling and synthesizing described in claim 1 to the gene order of the Talen recognition sequences pair；B) according to described Talen recognition sequences pair a), the expression vector of the gene knockout effect protein Talen of targeting Forkhead box n1 genes is built；C) carrier for obtaining step b) passes through liposome transfection, in the intracellular detection protein expression situations of 293T；D) carrier that step b) is obtained is passed through into in-vitro transcription, obtains the described mRNA for targeting knock out zebra fish Forkhead box n1 genes.The mRNA that the inventive method is obtained, can be used for knocking out zebra fish Forkhead box n1 genes, prepare zebra fish thymus development research model.Compared with prior art, low cost, design is simple, and success rate is high, has a significant effect. |
| priorityDate | 2014-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.