http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104357393-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
| filingDate | 2014-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104357393-B |
| titleOfInvention | A kind of isolated culture method of chicken intestinal epithelium gamma delta T cells |
| abstract | The present invention relates to a kind of isolated culture method of chicken intestinal epithelium gamma delta T cells.The present invention uses the discrete chicken intestinal epithelial cell of physical grinding fiting chemical enzyme process first, improves intestinal cell dispersion degree, shortens cell process time, it is ensured that the high activity of cell；The use of optimum density cell separation liquid, it is ensured that the purifying of gut epithelium lymphocyte；The use of magnetic bead secondary antibody separating method, it is ensured that target cell degree of purity higher；And by cell culture, it is found that the present invention is separately cultured the target cell high purity 90% of gained.The present invention is solved and is separately cultured a variety of drawbacks during chicken intestinal epithelium gamma delta T cells, so that for chicken intestinal epithelium gamma delta T cells culture and its biology and immunological investigation lay the foundation. |
| priorityDate | 2014-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.