| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_89f3efb8583f1229b9a555a3776c89ba |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-01019
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1074
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate |
2014-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43bc5461cbad3945d206e75f5d7f515f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67ee1e6e6dc9e7f6a1079402453d12da
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96f0effe1dd3230b02db00e0cc3013fc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a6021a0b6a559cad44b91ee2cb128a5 |
| publicationDate |
2015-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-104357371-A |
| titleOfInvention |
Genetically engineered bacterium for expressing beta cyclodextrin glycosyl transferase as well as construction method and use thereof |
| abstract |
The invention discloses a genetically engineered bacterium for expressing beta cyclodextrin glycosyl transferase as well as a construction method and a use thereof. The genetically engineered bacterium for expressing the beta cyclodextrin glycosyl transferase is genetically engineered bacterium E. coli BL21(DE3)/pET22-cgt obtained by transforming recombinant expression plasmid pET22-cgt which is obtained by inserting a beta cyclodextrin glycosyl transferase gene obtained by cloning into an expression vector pET22b(+) in host bacteria E.coliBL21(DE3). The genetically engineered bacterium for highly expressing beta-CGTase, constructed by the construction method disclosed by the invention, can be used for changing a culture medium of original microorganisms, separating thalli which appear in a production process of the bacteria to obtain relatively pure beta-CGTase, and directly preparing beta-CD by virtue of the beta cyclodextrin glycosyl transferase, so that the existing domestic beta-CD production process is radically improved to obtain high-quality beta-CD. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108384741-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108384741-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113584066-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111304094-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109706131-A |
| priorityDate |
2014-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |