http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104356238-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-20 |
| filingDate | 2014-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104356238-B |
| titleOfInvention | A kind of site specific immobilized method of recombinant protein A affinity ligand |
| abstract | The present invention provides a kind of site specific immobilized method of recombinant protein A affinity ligand.This method is that FGE identifications gene order is inserted into the gene order end of coding target protein by genetic engineering means, build the expression vector of the target protein, target protein is co-expressed in Escherichia coli with FGE again, realizes the intracellular aldehyde radical catalyzed conversion of target protein.Site specific immobilized is realized by the hydrazides group of dielectric surface and the selective reaction of protein surface aldehyde radical again.The method of the present invention can directly fix purpose recombinant protein from clasmatosis supernatant, and mild condition, step is simple, greatly reduces time and the cost of immobilization. |
| priorityDate | 2014-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.