http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104356234-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a712e6e7965beef77cd96f7cf92d3ca2 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-24 |
| filingDate | 2014-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_799f1d370945085992fa93f764f90208 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8eca3b03b0c4ff1cc8200e853c49790 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b455966d33e4ed99edb2bf3cb5d958d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae87faa01d2b55c2be46a133a1723060 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a7eb2630b7f73a7d7e6a3e31ac5f5ab8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d822621e5964e365d890477066891139 |
| publicationDate | 2015-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104356234-A |
| titleOfInvention | Method for preparing monoclonal antibody for treating psoriasis |
| abstract | The invention relates to a method for preparing a monoclonal antibody for treating psoriasis. The method is characterized by comprising the following steps: analyzing cell factors which are specifically expressed in psoriasis cells by using an isotope labeling and multi-dimensional liquid chromatogram-mass spectrum combination method, and separating and purifying the protein; immunizing a female BALB/c mouse of 6-8 week old by using the separated and purified cell factors which are specifically expressed in the psoriasis cells; if the antibody titer is greater than 1:10000, combining and screening a single-cell suspension of immune mice splenocyte and myeloma cells in the logarithmic phase, thereby obtaining hybridomas of a monoclonal antibody for secreting the cell factors specifically expressed in psoriasis cells; and injecting the hybridomas into the abdominal cavity of the BALB/c mice of 6-8 week old, collecting the ascites within about 10 days after the hybridomas are injected, and purifying the ascites by using a protein G/A column, thereby obtaining the relatively high-purity monoclonal antibody for treating psoriasis cell factors. |
| priorityDate | 2014-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.