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publicationDate 2015-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-104342387-A
titleOfInvention Preparation process of live bacillus sphaericus preparation
abstract The invention relates to a preparation process of a live bacillus sphaericus preparation. The preparation process comprises the steps that bacillus sphaericus is inoculated into a fermentation medium to be cultured after being subjected to primary and secondary seed enlarge culture; the quantity and conversion rate of bacillus are increased through fed-batch of a carbon source in the fermentation process, thus obviously increasing the unit yield and reducing the unit cost; after fermentation is completed, the fermentation liquor is concentrated by using a vacuum film concentrator and the soluble synergistic substances, growth-stimulated substances and bacteriostatic active substances in the fermentation liquor can be effectively recovered; the concentrated fermentation liquor is dried and granulated by multistage low temperature spray fluidized drying equipment, the granules are sorted and the fine powder is trapped by a cyclone separator and returns to be re-granulated, thus obtaining the bacillus sphaericus product, wherein the titer of each gram of the bacillus sphaericus powder is not less than 1000ITU/mg; the tail gas is treated and is discharged up to the standard. The preparation process has the advantages that the active substances in the fermentation liquor can be furthest retained; the product quality and the equipment utilization rate are high; the biological activity is better maintained; the waste gas and the wastewater are discharged up to the standard; the preparation process can be used for large-scale industrial production.
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