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filingDate 2014-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2015-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-104328098-A
titleOfInvention [Beta]-glucosidase, preparation method and application thereof
abstract The invention belongs to the fields of gene engineering technology and biological medicines, particularly relates to a [beta]-glucosidase, a preparation method and an application thereof and especially relates to an application in enzymic-method conversion for preparing ginsenoside 20(S)-Rg3. The [beta]-glucosidase is high-temperature-resistant and can maintain an enzyme activity to be constant almost at 70 DEG C for 3h. The [beta]-glucosidase has a strong conversion capability on ginsenoside Rb1. When being used for incubation with the ginsenoside Rb1 for 60 min, the [beta]-glucosidase enables the ginsenoside Rb1 to be converted almost into the ginsenoside 20(S)-Rg3. The method for preparing the ginsenoside 20(S)-Rg3 is less in intermediate product in an enzymatic conversion reaction and the ginsenoside 20(S)-Rg3 is high in purity after conversion.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110106158-A
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priorityDate 2014-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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