http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104327188-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
filingDate | 2014-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-08-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-08-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104327188-B |
titleOfInvention | A kind of fall webworms SUMO ABP dHC Cecropin A fusion proteins and its application |
abstract | The invention discloses a kind of fall webworms SUMO ABP dHC Cecropin A fusion proteins and its application, the fall webworms SUMO ABP dHC Cecropin A expressing fusion protein methods are as follows:PETSUMO ABP dHC Cecropin A expression vectors are built, supernatant Ni is collected after expression in escherichia coli, ultrasonication + Fall webworms SUMO ABP dHC Cecropin A fusion proteins are obtained after affinity purification, gradient elution.The present invention obtains fall webworms SUMO ABP dHC Cecropin A fusion proteins by gene recombination method, and high efficient expression is obtained in Escherichia coli, desired polypeptides reach big 95% purity by HPLC analyses, by mass spectral analysis, and molecular weight also coincide with prediction polypeptide molecular weight.After testing, polypeptide has obvious antifungal activity, and prepared desired polypeptides, its activity height, cost are low. |
priorityDate | 2014-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 93.