http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104313044-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4baf9c8f376c6c431fbd52ac964ad74b |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2014-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_abbcb778ed5aea6e8a7c5599e8cdf57e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_285fc72107173a1f5d9b9954a269bf7f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d7aa23b14b53fff6e1bfa473def7939 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_632b8c22d707417007479105faae0ac8 |
| publicationDate | 2015-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104313044-A |
| titleOfInvention | Zero-background cloning vector as well as preparation method and application thereof |
| abstract | The invention discloses a method for preparing a zero-background cloning vector, and application of the zero-background cloning vector. The method for preparing the zero-background cloning vector comprises the following steps: providing a DNA segment as shown in the sequence SEQ ID NO:6; performing first PCR amplification by taking pUC19 plasmid as a template and utilizing primers as shown in the sequences SEQ ID NO:7-8, thereby obtaining a pUC19 vector segment; performing homologous recombination on the DNA segment and the pUC19 vector segment, thereby obtaining a vector framework; converting the vector framework into competent cells, and extracting the plasmid, thereby obtaining a previous vector of the zero-background cloning vector; and performing enzyme digestion on the previous vector of the zero-background cloning vector by using EcoRV enzyme, thereby obtaining the zero-background cloning vector. The vector obtained by using the method can be directly used for connecting flat tail end products, the vector self-connection background is low, the positive rate of cloning is high, blue and white screening is not needed, rapid and effective cloning can be achieved within a short time, the cloning efficiency is high, and the effect is good. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112921050-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105200074-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106591341-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107760703-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106591341-B |
| priorityDate | 2014-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 173.