http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104312975-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-095 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2014-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104312975-B |
| titleOfInvention | A kind of external dimensional culture model of glioma stem cells and application |
| abstract | The invention belongs to cell biologies, and in particular to a kind of external dimensional culture model and application for being used to study glioma stem cells vascular mimicry.The preparation of the external dimensional culture model carries out in accordance with the following steps：Three-dimensional collagen scaffold is placed in DMEM culture mediums after impregnating 6 24 hours and taken out, the extra DMEM sticked on the three-dimensional collagen scaffold of removal must pre-process three-dimensional collagen scaffold, the three-dimensional collagen scaffold of pretreatment is placed in tissue culture plate, by the glioma stem cells plantation of balling-up in the three-dimensional collagen scaffold of pretreatment, after 37 DEG C stand 26 hours, add in endothelium culture medium, 37 DEG C of cultures 24 days are to get to for studying the external dimensional culture model of glioma stem cells vascular mimicry.The external various operating process of dimensional culture model carry out at room temperature, various dyeing can be carried out in situ, including immunohistochemistry, immunofluorescence dyeing；It can carry out Electronic Speculum detection；RT PCR and Western Blot detections are carried out after can directly digesting. |
| priorityDate | 2014-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.