http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104311685-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08B37-00 |
| filingDate | 2014-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104311685-B |
| titleOfInvention | A kind of spirulina polysaccharide and extracting method thereof |
| abstract | The present invention relates to a kind of spirulina polysaccharide and extracting method thereof, comprise the following steps: after Spirulina powder suspension multigelation breaking cellular wall, centrifugal acquisition cell breakage liquid 1 and cell debris；Adding ammonium sulfate in cell breakage liquid 1 is 50% to its saturation, after salt precipitation, and centrifugal segregation phycobniliprotein, it is thus achieved that supernatant, supernatant is mixed with cell debris, it is thus achieved that cell breakage liquid 2；Hot water extraction is used to obtain spirulina polysaccharide crude extract；Crude extract is joined in ethanol/ammonium sulfate double-aqueous phase system and extract, it is thus achieved that there is the lower phase solution of the spirulina polysaccharide of higher degree；Lower phase solution is after dialysis desalination, with Sephadex G 150 chromatographic column and DEAE Sephadex A 50 anion-exchange column eluting, it is thus achieved that pure spirulina polysaccharide solution；After lyophilization, it is prepared as pure spirulina polysaccharide finished product.This extracting method can avoid tradition to go the troublesome operation step of removing protein, low cost, yield height, and purity of polysaccharide and activity high, it is adaptable to intermittent and large-scale production is processed. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110183544-A |
| priorityDate | 2014-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.