http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104304238-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2014-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104304238-B |
| titleOfInvention | The Embedding drying cryopreservation method of downy grape stem apex |
| abstract | The invention discloses the Embedding drying cryopreservation method of a kind of downy grape stem apex, it is characterized in that: comprise the following steps: (1) stem apex Embedding drying Ultra-cryofreezing preservation: take the tissue cultured seedling that subculture axillalry bud is full, at illumination cold acclimation, after strip tissue cultured seedling axillalry bud stem apex, make stem apex embedding ball, to embed ball preculture in sucrose solids culture medium again, dried stem apex embedding ball is placed in freezing 24 more than h in liquid nitrogen；(2) after thawing and recover, regeneration is cultivated: after stem apex embedding ball freezen protective, thaw, and then takes embedding ball stem apex and is inoculated on recovery media and carries out renewal cultivation and successive transfer culture obtains regenerating stem apex;(3) root culture: take the regeneration stem apex in step 2 and be placed in root media and carry out root culture；(4) acclimatization and transplants: the root cultivating the Seedling of taking root of 30 40d is cleaned, KMnO 4 Leaching root, transplant and water with 1/8MS nutritional solution in the nutritive cube fill sterilizing Vermiculitum, be then placed in watering antibacterial after 23 d cultivate in culturing room, transfer to fill after overground part leaf color is deepened and the nutritive cube of Nutrition Soil continues greenhouse take exercise, growing about 2 ~ 3 months, the spring in next year transplants land for growing field crops. |
| priorityDate | 2014-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.