http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104293884-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-527 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 |
| filingDate | 2014-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104293884-B |
| titleOfInvention | A kind of acetyl CoA contents measures test kit and method thereof |
| abstract | The invention discloses a kind of acetyl CoA contents and measure test kit and method thereof, this test kit includes: reagent one, is made up of Tris HCl, polyvinylpyrrolidone, mercaptoethanol, Phenylmethanesulfonyl fluoride and glycerol；Reagent two, is made up of malic dehydrogenase；Reagent three, is made up of citrate synthase；Reagent four, is made up of malic acid and oxidized form of nicotinamide-adenine dinucleotide；Reagent five, is made up of Tris HCl.Malic dehydrogenase can be catalyzed malic acid and oxidized form of nicotinamide-adenine dinucleotide generates oxaloacetic acid and DPNH, citrate synthase can be catalyzed S-acetyl-coenzyme-A and oxaloacetic acid generates citric acid and coenzyme A, utilize the coupling reaction of malic dehydrogenase and citrate synthase, the generating rate of acetyl CoA contents and prototype nadide is directly proportional, and under 340nm, the climbing speed of light absorption value has reacted the height of acetyl CoA contents.The present invention is easy and simple to handle, and detection sensitivity is high, and the response rate is high, significantly reduces reagent cost, present invention also simplifies detecting step, measures more simple and efficient. |
| priorityDate | 2014-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 661.