http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104293833-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-873 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2014-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104293833-B |
| titleOfInvention | A kind of special targeting vector of Sp110 macrophages and recombinant cell mediated based on TALEN |
| abstract | The invention discloses a kind of special targeting vector of Sp110 macrophages and recombinant cell mediated based on TALEN, the M S sites fixed point that gene targeting acquisition is carried out to bovine fetal fibroblast knocks in the bovine fetal fibroblast of Sp110 genes.Utilize the MSR1 promoters in targeting vector so that the Sp110 that knocks in of fixed point and only normal transcription and expression in the peripheral blood macrophage of ox.The present invention, by targeting vector and TALEN site specific nucleic acid nickase expression vector cotransfection bovine fetal fibroblasts, is screened and verified the positive colony cell for obtaining target practice by PCR and Southern Blotting using the method for electroporation.Ox enucleation oocyte is moved into by nuclear donor of the positive colony cell, transgenic cloned embryos are obtained.Transgenic cloned embryos are transplanted in the acceptor cattle uterus of heat, the transgene clone ox that final production is survived. |
| priorityDate | 2014-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 123.