http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104293757-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-02004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-06 |
| filingDate | 2014-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104293757-B |
| titleOfInvention | A kind of aldolase and encoding gene thereof and application |
| abstract | The invention discloses a kind of aldolase and encoding gene thereof and application.The aminoacid sequence of aldolase is as shown in SEQ ID No.1, and the base sequence of its encoding gene is as shown in SEQ ID No.2.Described application is the application in producing statins drug midbody of this aldolase.The present invention is on the basis of Thermophilic Bacteria wild type aldolase, its encoding gene is carried out random mutation superposition rite-directed mutagenesis, obtaining double mutant gene, the aldolase that this double mutant gene is expressed not only has possessed the anti-substrate degeneration ability of wild type aldolase, and its catalysis activity is also greatly improved；Utilizing this enzyme catalysis acetaldehyde and 2-Chloro-1-ethanal generation aldol reaction, its catalytic efficiency improves 3 times than wild type aldolase；Show that the aldolase of the present invention has good prospects for commercial application. |
| priorityDate | 2014-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 512.