http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104273038-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
filingDate | 2014-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104273038-B |
titleOfInvention | The tissue culture method of a kind of Mount Huang Luan Shu |
abstract | The present invention relates to the tissue culture method of a kind of Mount Huang Luan Shu, comprise the steps, the preparation of (1) Mount Huang Luan Shu leaf extract; (2) just for Fiber differentiation: Mount Huang Luan Shu stem explants is inoculated in and just cultivates on inducing culture, just for being added with Mount Huang Luan Shu leaf extract in inducing culture; (3) shoot proliferation is cultivated: the stem section of the first Mount Huang Luan Shu aseptic seedling obtained for Fiber differentiation or stem apex aseptic inoculation are cultivated in subculture multiplication medium, be added with Mount Huang Luan Shu leaf extract in subculture multiplication medium; (4) plantlet in vitro is taken root: the aseptic tufted seedling of Mount Huang Luan Shu that Multiplying culture obtains is cut into individual plant and is inoculated on root media, be added with Mount Huang Luan Shu leaf extract in root media; (5) domestication of test-tube plantlet and transplanting.Adopt this programme can shorten cycle duration 15%, cost-saving 20%, be applicable to factorial praluction. |
priorityDate | 2014-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.