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filingDate 2014-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79dcfb9a44b8d12f2b8858c54c0c3486
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publicationDate 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-104255403-B
titleOfInvention Screening method for glyphosate-resisting medicago sativa plants
abstract The invention mainly provides a medicago sativa plant resistant to herbicide and a method, particularly relates to a method for screening medicago sativa plants resistant to the herbicide by utilizing chemical mutagen EMS (ethyl methane sulfonate) for mutagenesis of medicago sativa, and discloses a culture medium screening method for glyphosate-resisting medicago sativa plants. The method is characterized by including the following steps: (1) germinating seeds by an MS culture medium; (2) performing dosage screening; (3) subjecting medicago sativa seeds to mutagenesis by median lethal dose of the EMS; (4) screening the glyphosate-resisting medicago sativa plants; (5) rescreening the resistant plants. The method has the advantages of being simple and easy to implement, taking effect fast and capable of allowing single plants of herbicide-resistant medicago sativa to be obtained within a short time. A mutagenesis way is adopted. The chemical mutagen EMS is utilized to mutate the medicago sativa seeds to generate point mutation, the single plants of the herbicide-resistant medicago sativa are screened by two ways of the culture medium and soil screening, new germplasm resources are provided for conventional breeding, and certain references are provided for herbicide-resistant breeding of other plants.
priorityDate 2014-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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