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filingDate 2014-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-104250665-B
titleOfInvention No. 5 microspecies RTQ-PCR detection primers of Ralstonia solanacearum and method
abstract No. 5 microspecies RTQ PCR detection primers of Ralstonia solanacearum and method, sequence is: RS72F:5'ATGGATAAAGGGTTCGTGGTG 3';RS312R:5'CAGGCTCAGCGAGATTGC 3'.The present invention is different from the specific fragment design of other Ralstonia solanacearum microspecies based on No. 5 microspecies of Ralstonia solanacearum and has screened a pair specific primer, utilize the fluorescent quantitative PCR detection method of this design of primers mulberry Ralstonia solanacearum simultaneously, there is the best, highly sensitive feature, and substantially reducing detection required time, sample treatment only needs 4~5h to obtaining a result.It is applicable to the early diagnosis of mulberry bacterial wilt.
priorityDate 2014-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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