http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104237519-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56916 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 |
| filingDate | 2014-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104237519-B |
| titleOfInvention | A kind of DASELISA immunization detecting P.stwartii subsp.stewartii |
| abstract | Detect a DASELISA immunization for P.stwartii subsp.stewartii, belong to immunoassay field.The present invention P.stwartii subsp.stewartii NCPPB? 449(imports and exports inspection and quarantine bureau from Hunan Province) immune BALB/c mouse, through immunity, merges, screens to obtain 5 strain P.stwartii subsp.stewartii monoclonal antibodies.5 strain antibodies mark HRP respectively and match between two with this thalline.With No. H, 11C2(monoclonal cell strain) for coated antibody, No. I, 10B1(monoclonal cell strain) be enzyme labelled antibody, with NCPPB? 449 thalline are that Sandwich ELISA set up by standard items.LOD is 1.5 × 10n 4 cfu/mL, the range of linearity 4.57 × 10n 5 ~ 1.11 × 10n 8 cfu/mL.The monoclonal antibody that the present invention's physicochemical property height is homogeneous, specificity good, can prepare in a large number, the sandwich method set up is highly sensitive, cost is low, with no cross reactions such as pseudomonas syringae spot pvs oryzae and oryzicola, in corn seed, the detection of P.stwartii subsp.stewartii provides analysis means rapidly and efficiently. |
| priorityDate | 2014-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.