http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104232767-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A90-40 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2014-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104232767-B |
titleOfInvention | Ralstonia solanacearum rolling circle amplification detection method |
abstract | The present invention relates to a kind of Ralstonia solanacearum detection method, be more particularly to a kind of use padlock probe that Ralstonia solanacearum is carried out the detection method of rolling circle amplification, belong to crops and prevent and cure diseases and enter and leave the border plant quarantine category.The present invention utilizes the feature that padlock probe can be combined with template DNA complementation, Ralstonia solanacearum specific gene fragment sequence designs padlock probe, hyper-branched rolling circle amplification is carried out by primer, set up Ralstonia solanacearum hyper-branched rolling circle amplification detection method, the tradition Ralstonia solanacearum detection time can be shortened, it is ensured that the specificity of detection and sensitivity.And this method can shorten the cycle of tradition detection of pathogens, improve clearance speed.This intercepts and captures recall rate, Exotic pests invasive ability of improving the precaution for improving large agricultural product epidemic situation that enters the territory, and accelerates the clearance speed of China's port cargoes imported and exported, reduces entreprise cost and is all significant. |
priorityDate | 2014-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.