| abstract |
The present invention relates to a kind of method of production natural killer cells (hereinafter, referred to as " NK cells "), by the NK cells of this method production and the composition for treating cancer and infectious diseases comprising the NK cells.The present invention provides a kind of method producing NK cells, which keeps high cytoactive and cytotoxicity and show high curative effect to cancer and infectious diseases, and can in vitro be cultivated with high efficiency and high concentration.In addition, thus preventing the undue growth of cell, optimum state being kept a cell in so a kind of cultural method that holding cell concentration is in constant level for producing NK cells.Particularly, even if thaw after cell is frozen, the function of cell will not be damaged, and NK cells can keep high cytoactive and cytotoxicity.Therefore, it is possible to easily store and provide NK cells with liquid condition or freezing state, without additional processing procedure. |