http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104198713-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56983 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-78 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 |
| filingDate | 2014-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104198713-B |
| titleOfInvention | A kind of method of tomato spotted wilf virus in quick detection thrips body |
| abstract | The invention discloses the method for tomato spotted wilf virus in a kind of quick detection thrips body, to be measured 1 or several thrips being placed into finishes on the nitrocellulose filter of separation, add 2 μ l sample treatment buffer, with liquid transfer gun head, thrips is ground in damping fluid, process different polypide sample successively, the same absorption 2 μ l positive and negative controls point are on nitrocellulose filter; After film drying, close 1h with 5% skimmed milk power of PBST buffer, then the primary antibodie adding confining liquid dilution cultivates 1h, washes film afterwards 3 times; Add two anti-cultivation 1h of confining liquid dilution, then wash film 3 times; Add nitrite ion colour developing 15min, discard nitrite ion and add the reaction of distilled water color development stopping, can interpretation of result be carried out.The present invention is easy to operate, quick, measurement result has good stability, reappearance and accuracy, can meet the detection needs of tomato spotted wilf virus in thrips body, lays a good foundation for avoiding the harm of tomato spotted wilf virus as far as possible. |
| priorityDate | 2014-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.