| abstract |
The invention discloses an enzyme-linked immunospot test method for T lymphocytes. The method comprises the following steps: by adopting a T-SPOT.TB kit produced in British Oxford Immunotec Company, taking 5ml of sodium citrate anticoagulation venous blood, separating mononuclear cells of peripheral blood and placing the mononuclear cells on a gamma-interferon antibody coated porous plate, adding an early secretory antigenic target (ESAT-6) of tuberculosis specific antigen and a culture filtrate protein (CFP-10) to serve as stimulus antigens, culturing in an incubator for 24 hours, washing off T lymphocytes with antigen sensitization effects, adding a biotin labeled second antibody, performing enzyme linked colorimetry, forming spots at secreting positions of cell factors, and detecting the number of the T lymphocytes with the tuberculosis specific antigen sensitization effects by recording the spot number. The method disclosed by the invention contributes to early screening of culture negative pulmonary tuberculosis. |