http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104195223-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-13 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2014-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104195223-B |
titleOfInvention | A kind of method for the identification of resistance to iron deficiency apple rootstock |
abstract | The invention discloses a kind of method for the identification of resistance to iron deficiency apple rootstock. The method comprises the steps: 1) apple rootstock to be measured and the resistance to iron deficiency apple rootstock of standard are carried out respectively to iron deficiency processing, extract total RNA; 2) adopt, for the gene probe of resistance to iron deficiency, total RNA is carried out to dot hybridization; 3) with ImageScannner scanning, and analyze with ImageMaster, obtain the expression of the gene of resistance to iron deficiency described in the resistance to iron deficiency apple rootstock of described apple rootstock to be measured and described standard; 4), if the expression of the gene of resistance to iron deficiency described in described apple rootstock to be measured is not less than the resistance to iron deficiency apple rootstock of described standard, described apple rootstock to be measured is or candidate is resistance to iron deficiency apple rootstock. Experiment showed, that the inventive method has with low cost and efficient advantage, and overcome the limitation of resistance to iron deficiency apple rootstock conventional herd breeding, on gene level, carry out seed selection, for good basis is established in the breeding of gene expression marker assisted selection. |
priorityDate | 2014-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.