http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104186460-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
| filingDate | 2014-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104186460-B |
| titleOfInvention | The method of complete freezing preservation and the intracellular nucleic sugar body new polypeptide chain compound that thaws |
| abstract | The invention discloses a kind of complete freezing preservation and the method for the intracellular nucleic sugar body new polypeptide chain compound that thaws. The method is in the cell culture fluid of normal growth, to add translation to extend inhibitor, extension inhibitor to be translated useful effect is in RNC, suppress after the carrying out of translation extension, cell is cooled to 0～4 DEG C, clean cell and add in cell with the PBS solution that is chilled in advance 0～4 DEG C again, snap frozen in liquid nitrogen, ultralow temperature is preserved, in thawing on ice, then extracting RNC. The present invention translates extension inhibitor by employing and effectively fixes ribosomes, makes it to be difficult for dissociating, and intracellular translation situation is fixed up; Suddenly freeze method freezing preservation RNC long term storage and transport effectively by liquid nitrogen again, and the RNC extracting after thawing and the RNC of fresh cell extraction are as good as, therefore the present invention has broken through the restriction in time and space, for studying RNC and carrying out RNC commerce services and provide convenience. |
| priorityDate | 2014-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.