http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104178557-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2013-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104178557-B |
| titleOfInvention | The detection method and its primer of leukemia fusion gene, probe and genetic chip |
| abstract | The invention discloses a kind of method that multiple RT-PCR Polymorphism chip detects leukemia fusion gene, step includes：1）Extract sample cell total rna；2）RNA specific reverse transcriptases are cDNA；3）Multiplex RT-PCR amplification cDNA；4）With the genechip detection pcr amplification product of the specific probe of the leukemia fusion gene containing detection.The invention also discloses probe, primer and the genetic chips used in the above method.The present invention uses a pipe Nest multiplex PCR, the specific fusion genetic fragment that may contain in amplified sample, pass through gene chip hybridization and chip scanning image analysis again, obtain fusion testing result, it is detected while the specific fragment for so realizing 27 kinds of 15 leukemia fusion genes or more different fusion forms, to substantially increase the detection efficiency of leukemia fusion gene, be conducive to large-scale screening. |
| priorityDate | 2013-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.