http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104178465-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0016 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-01004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-01003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 |
| filingDate | 2014-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104178465-B |
| titleOfInvention | A kind of method that glutamte dehydrogenase is extracted from cattle liver |
| abstract | The invention discloses a kind of method that glutamte dehydrogenase is extracted from cattle liver, by the glutamate dehydrogenase enzyme extract being prepared using cattle liver as raw material, the extraction purification that glutamte dehydrogenase is carried out with gel chromatography technology using being concentrated by ultrafiltration, it instead of the complicated technology extracted using a large amount of organic solvents or inorganic salt solution etc., on the one hand make extraction process simpler easy to operate, greatly reduce production cost, on the other hand the permeate repetition recycling produced will be concentrated by ultrafiltration, the water consume of every kilogram of raw material is reduced to less than 2.0 kilograms, avoid the discharge of waste water, it is a kind of environmentally friendly production technology.In addition, the phosphate buffer of precooling is used and supplemented during smashing to pieces, make to smash the process progress under low temperature all the time to pieces, it is ensured that the activity of glutamte dehydrogenase, gained glutamte dehydrogenase enzyme activity reaches more than 9000U/mg, and yield reaches more than 21%. |
| priorityDate | 2014-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.