http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104178463-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_70fbe08b7c540157200696115221f654 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y114-14003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0073 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
| filingDate | 2013-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5eb3b58823135a18cb06bf45397d0cf2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12a005376d9c91fbfd3d911fbf5d0a27 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_661783ec131ddd19124da512847bc490 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db2ec3f2b2ef5204b3c083d66705495d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dee6b63b468683cab43b474f6e27142f |
| publicationDate | 2014-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104178463-A |
| titleOfInvention | Method for preparing enhanced monomeric bacterial luciferase luxAB |
| abstract | The invention discloses a method for preparing an enhanced monomeric bacterial luciferase luxAB. The method comprises the following steps: obtaining a target gene; constructing a recombinant expression vector; obtaining an expression strain containing a recombinant expression plasmid; inducing the expression of a target protein, and purifying the protein; and detecting the activity of the purified enhanced monomeric bacterial luciferase. The unique advantages of fusion type luciferase are utilized to fuse luxA and luxB heterodimer luciferase gene, nine fluorescence intensity enhanced mutants are obtained by screening through error prone PCR mutation and chemical random mutation, a shuffling technology is carried out to obtain enhanced monomeric bacterial luciferase gene luxAB, the enhanced monomeric bacterial luciferase gene luxAB is cloned into an expression vector pET28a, the obtained vector is transformed into a BL21 (DE3) strain to express, a mild breaking technology is used to lyse cells, and an expressed product is purified to obtain the enhanced monomeric bacterial luciferase. The method has the advantages of simple process, short growth cycle, low cost, easy purification, small enzyme activity loss, stable performances of the above enzyme, and important actual application values. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112980861-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109734815-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113563484-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113563484-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2020339964-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111315875-A |
| priorityDate | 2013-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 78.