http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104164488-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2014-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104164488-B |
| titleOfInvention | A kind of nucleic acid constant-temperature amplification method that single primer causes |
| abstract | The invention belongs to nucleic acid detection technique field, be specifically related to a kind of nucleic acid constant-temperature amplification method that single primer causes.The shortcomings such as the technical problem to be solved is to provide a kind of nucleic acid constant-temperature amplification method that single primer causes, it is to avoid in traditional Constant Temperature Detection method of prior art, design of primers is complicated, and testing cost is high.The technical scheme that the present invention provides is a kind of nucleic acid constant-temperature amplification method that single primer causes, by the design of primer sequence makes primer self-complementary after matching with target nucleic acid match, form loop-stem structure then to depart from from target nucleic acid, under nicking enzyme and polymerase act on jointly, constantly produce nucleotide fragments.Technical solution of the present invention can complete under isothermal conditions, just can be implemented just with the equipment that can keep steady temperature, easy to operate；Amplification procedure only needs a primer, so that detection scheme is more simple and easy to do, improves the efficiency of nucleic acid amplification and detection. |
| priorityDate | 2014-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 292.