| abstract |
The invention provides a polymer brush-modified magnetic composite microsphere and its preparation method and application. The polymer brush-modified magnetic composite microsphere sequentially includes: a polymer core, a superparamagnetic nanoparticle shell and a polymer core. The polymer brush is directly grafted onto the shell of the superparamagnetic nanoparticle by the carboxyl group-containing water-soluble unsaturated monomer through atom transfer radical polymerization. By grafting polymer brushes on the surface of magnetic nanoparticles, the specific surface area and protein binding sites of the microspheres can be greatly increased, which improves the chance of protein and material contact. In addition, it is also convenient to further graft specific functional groups on the polymer brush, improve the ability of the microspheres to specifically bind to the separation target, and achieve a finer separation purpose. The preparation method is simple, improves the stability, operability and environmental friendliness of the reaction system, and is convenient for popularization and application. |