http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104131055-B
Outgoing Links
Predicate | Object |
---|---|
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-405 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 |
filingDate | 2014-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104131055-B |
titleOfInvention | Preparation method for phycoerythrin ACE inhibitory peptide |
abstract | The invention discloses a preparation method for a phycoerythrin ACE inhibitory peptide. The method includes the steps of: extracting phycoerythrin; adding pepsin to conduct enzymolysis and then adding trypsin to perform enzymolysis; dissolving the freeze-dried powder subjected to enzymolysis in pure water, loading the obtained solution to a Sephadex G-15 gel column, collecting the highest activity peak as a phycoerythrin ACE inhibitory peptide component, and further loading the obtained component to a high performance liquid chromatogram ZORBAX 300SB-C18 to perform separation so as to detect phycoerythrin ACE inhibitory peptide fragments in the component and can realize preparation of high purity phycoerythrin ACE inhibitory peptide at the same time. The method provided by the invention employs pepsin and trypsin stepwise enzymolysis to prepare the ACE inhibitory peptide, can avoid inactivation of the active peptide due to degradation by gastrointestinal digestive fluid after intake by the human body, also reduces the cost, has a simple process, and can realize industrial production. The phycoerythrin ACE inhibitory peptide derives from natural vegetable protein, has a small molecular weight, is stable, safe, and easy to absorb by the human body. |
priorityDate | 2014-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 98.