http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104130977-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2014-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104130977-B |
| titleOfInvention | A kind of screening anti-tumor medicine cell model and its application |
| abstract | A kind of screening anti-tumor medicine cell model and its application, belong to technical field of bioengineering.Screening anti-tumor medicine cell model be separated from glioblastoma, the site-directed integration tumor stem cell of reporter gene, DNA constructions containing A-B-C-D structures, wherein：A, D are glioblastoma stem cell molecular marker gene promoter and downstream sequence, and B is reporter sequences, and C is puromycin resistance gene puro sequences；The dry extracellular molecule marker gene of glioblastoma is to include Nestin genes；Reporter gene includes green fluorescence protein gene GFP or red fluorescent protein gene RFP and luciferase reporter gene luciferase.The cell model of the antineoplastic is used to screen the material of cytotoxicity and induction differentiation.Beneficial effect is：1st, the adverse effect of traditional cell transgenic method is avoided, testing result can correctly reflect drug effectiveness.2nd, the compound of resisting tumour stem cells is obtained. |
| priorityDate | 2014-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.