http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104099391-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2013-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_48f5017b8b38fd6d1a18f519735a4f6f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21d98518490d3bfc3e64218806601ce1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_566551777c9a8349c0ba7853d0cc291d |
| publicationDate | 2014-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104099391-A |
| titleOfInvention | Method for separating RAVE compound from Saccharomyces cerevisiae cells by using FLAG label |
| abstract | A method for separating an RAVE compound from Saccharomyces cerevisiae cells by using an FLAG label relates to modification and purification of an RAVE compound protein in Saccharomyces cerevisiae, and belongs to the fields of the molecular biology and proteomics. The invention provides a method for separating the RAVE compound from a Saccharomyces cerevisiae recombinant strain by using the affinity chromatography principle of the FLAG label, and lays a solid foundation for the subsequent researches of the structure of the compound. The affinity chromatography principle of the FLAG label is used to separate easily biodegradable multimeric regulatory protein RAVE compound from a large amount of a cell homogenates supernatant, so the concentration and the purity of the RAVE compound are greatly improved; the FLAG label only contains 8 amino acids, and has a very small influence on the structures and the functions of proteins, so the activity of the separated RAVE compound is maximally guaranteed; and a series of proteins interacting with the RAVE compound by separating through the FLAG label affinity chromatography, and the identification by combining with mass spectrum can enrich the research data of RAVE compound related proteomics. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106636043-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106636043-B |
| priorityDate | 2013-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.