http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104087682-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2014-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104087682-B |
titleOfInvention | The method utilizing genechip detection astragaloside induction RMMVECs gene expression profile |
abstract | The present invention provides a kind of method utilizing genechip detection astragaloside induction RMMVECs gene expression profile, comprises the following steps: (1) preparation purification of rat myocardium microvascular endothelial cells RMMVECs;(2) cultivating secondary RMMVECs, be dissolved in by astragaloside in maintenance culture medium, concentration is 10 μ g/ml, at 37 DEG C, 5%CO 2 Under conditions of, continue to cultivate 9h by RMMVECs;Deng nine steps;The present invention has successfully cultivated RMMVECs, and utilize biochip technology fully to demonstrate from gene expression dose, improve blood capillary relax contracting activity amplitude Effective Component of Chinese Medicine Ast or Ber energy two-ways regulation RMMVECs relevant cell factor expression, maintain the balance of cytokine secretion, maintain the normal physiological function of MVECs, thus blocked the virulence factor injury to capillary endothelium, play the effect for the treatment of disease. |
priorityDate | 2014-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 142.