http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104087601-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_83d922185731caa1dcb9bce52668ad70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
| filingDate | 2014-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_110147abafa74ec73b4b7450dd9e026b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e0865c81768d8784d32e80c41c2bacf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f7644a46946ffb3dd16afcab23e80a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a5809edb9f0b6238c91652f99948797 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc9ddfcceddc2526186f6c47f7ca238f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27707ee63e83c9fda1a7244fa56550e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b60f8fa304c291b43a8d99ba61c36d46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67ec3b5c3f1bcc8c13f09c51af312062 |
| publicationDate | 2014-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104087601-A |
| titleOfInvention | Method for producing chirally pure S-acetoin |
| abstract | The invention relates to a method for producing chirally pure S-acetoin. The method comprises the following steps: carrying out coexpression on a gene of diacetyl reductase (dar) and a gene of glucose dehydrogenase (gdh) in Escherichia coli, converting and producing chirally pure S-acetoin in the presence of a recombinant Escherichia coli rest cell used as a biocatalyst and diacetyl and glucose used as substrates. The diacetyl reductase has the characteristic that diacetyl is catalytically produced into the chirally pure S-acetoin, and the glucose dehydrogenase can provide the coenzyme NADH required for the diacetyl reductase, the yield of S-acetoin can reach up to 28g / L and the optical purity of S-acetoin is 99.7%. The method disclosed by the invention has the advantages of simple medium, convenience in operation process and low cost, the product S-acetoin is conveniently separated, the complex chiral separation step does not be needed, the intracellular coenzyme regeneration is achieved and the method has industrial application prospects. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105969812-A |
| priorityDate | 2014-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 83.