http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104087546-B
Outgoing Links
Predicate | Object |
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classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-62 |
filingDate | 2014-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104087546-B |
titleOfInvention | A kind of method of engineering bacteria and preparation chloro-3, the 5-dihydroxy hecanoic acid t-butyl esters of (3R, 5S) 6- |
abstract | The invention discloses a kind of engineering bacteria and the method for preparation chloro-3, the 5-dihydroxy hecanoic acid t-butyl esters of (3R, 5S) 6-, this engineering bacteria comprises alcohol dehydrogenase gene, glucose dehydrogenase gene, carbonyl reductase gene;The method includes culturing engineering bacterium and induces enzyme to express, and centrifuging and taking cell is resuspended with buffer, it is thus achieved that resting cell suspension;In resting cell suspension, add chloro-3, the 5-dicarbapentaborane hecanoic acid t-butyl esters of 6-, glucose and isopropanol react, after having reacted, from reactant liquor, separate purification obtain chloro-3, the 5-dihydroxy hecanoic acid t-butyl esters of (3R, 5S) 6-.The present invention realizes from 6-chloro-3,5-dicarbapentaborane hecanoic acid t-butyl ester is to (3R, 5S) 6-chloro-3, one stepization of 5-dihydroxy hecanoic acid t-butyl ester produces, the technique simplifying chloro-3, the 5-dihydroxy hecanoic acid t-butyl esters of (3R, 5S) 6-, avoid the interpolation of extra coenzyme, reduce production cost. |
priorityDate | 2014-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 295.