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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-48
filingDate 2014-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa85d2f67751f68d455e9abcaad06dca
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2764d1813846274844eea2cd09b5767
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publicationDate 2014-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-104059964-A
titleOfInvention Method For Detecting Mercury Ions In Solution, And Kit
abstract The invention provides a method for detecting mercury ions in a solution, and a kit. The method comprises: 1) mixing the solution with single-stranded DNA containing thymine to form a mixture so that the single-stranded DNA containing the thymine forms double-stranded DNA through T-Hg2+-T base pairs wherein at least one deoxyribonucleotide of the single-stranded DNA containing the thymine is connected with a signal generating molecule; 2) contacting the double-stranded DNA obtained from the step 1) with exonuclease specifically different from the double-stranded DNA so that the exonuclease cuts the double-stranded DNA to release the deoxyribonucleotides connected with the signal generating molecules; and 3) detecting the signal generating molecules connected with the deoxyribonucleotides released in the detection step 2). The method has high specificity and sensitivity, does not need fixed DNA, is convenient to operate and can realize rapid detection.
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priorityDate 2013-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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