http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104045707-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-605 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-605 |
| filingDate | 2013-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104045707-B |
| titleOfInvention | Purification method of teduglutide |
| abstract | Relating to the field of medicine technologies, the invention discloses a purification method of teduglutide. The method includes: dissolving a teduglutide crude product with an acetonitrile-acetic acid mixed water solution, then adding water to perform dilution till a volume percent sum of acetonitrile and acetic acid of 10%-30%, then employing RP-HPLC to conduct gradient elution, separating a teduglutide flow part with purity of over 95% for standby use, separating a teduglutide flow part with purity of 90%-95% to undergo elution again, mixing the teduglutide flow part with purity of over 95%, adjusting the volume percent of acetonitrile to less than 15% and the pH value to 6-7, and then conducting centrifugation to obtain a purified solid product. According to the invention, the acetic acid-acetonitrile mixed dissolution system is employed to conduct pretreatment on the teduglutide crude product, then a proper flow phase is employed to conduct RP-HPLC gradient elution, and finally by means of salting-out and desalination purification, the high quality teduglutide pure product can be obtained. Compared with the existing RP-HPLC purification methods, the method provided by the invention improves the purification efficiency and yield. |
| priorityDate | 2013-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 77.