http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104041416-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
filingDate | 2014-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104041416-B |
titleOfInvention | A kind of method utilizing Chinese yew Callus of Leaf to produce taxol |
abstract | The invention provides a kind of method that taxus callus produces taxol, with ' Man Diya ' Chinese yew blade for explant, through sterilization, sterilizing, inoculation, after cultivating a period of time, produce callus in blade wound, continue to carry out Multiplying culture to callus.Get Chinese yew Callus of Leaf grind and use ethyl acetate ultrasonic extraction 3 times, collect organic facies, use rotary evaporator decompression distillation after filtering, be dissolved in a small amount of methyl alcohol, obtain the methanol solution containing taxol.The present invention's application tissue culture technique overcome Chinese yew plant strain growth slowly, the problem of taxol chemosynthesis complexity.Minimal medium used, based on SH medium, is aided with the compositions such as 2,4-dichlorphenoxyacetic acid, L-PROLINE, sucrose; Thering is provided basis, environmental friendliness, abundant raw material for carrying out batch production cells produce taxol, the problem that market is large to taxol supply breach can be alleviated very well. |
priorityDate | 2014-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 71.