http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104016864-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_53b4d53983adbf56dd0e7d0bf96f8ce5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C2601-14 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C67-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C67-48 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C67-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C69-732 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C67-48 |
| filingDate | 2014-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a3f36588148f5918f5bdfb7bb11e549 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb298f17c2e4af389b7811e4c188162f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27a7a1c370929e2a44a06a504d2ddb28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c566e9832e058b24d4d2b273e4c89c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47275b4c01231cfccf413bc294c6e9e1 |
| publicationDate | 2014-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104016864-A |
| titleOfInvention | A method for preparing chlorogenic acid and neochlorogenic acid from prickly ash leaves |
| abstract | The invention discloses a method for extracting chlorogenic acid and new chlorogenic acid from Zanthoxylum bungeanum leaves, which is characterized in that the dried Zanthoxylum bungeanum leaves are crushed, the average particle size is 400-600 μm, and the concentration is 50-30 μm according to the ratio of material to liquid: 1 g: 8-30 mL. In 80wt% methanol, shake at room temperature for 24-36 hours, separate the supernatant, concentrate and dry to obtain methanol extract; disperse the above-mentioned methanol extract in distilled water, the ratio of solid to liquid is 1g:10-30mL, and the volume ratio is 1: 2 Add ethyl acetate and shake and extract 2 to 3 times, collect the aqueous phase extract and concentrate and dry it, with a yield of 19.8 to 25.8%; the dried sample is applied to a reverse-phase resin SciBioChem MCI-GEL chromatography column with a packing height of 20 cm and a column diameter of 3.2cm, eluted with methanol-water gradient, the concentrations are 0wt%, 10wt%, 20wt%, 30wt%, 40wt%, 50wt% methanol, and the elution flow rate is 1-2mL/min. The 10wt% and 20wt% methanol eluents were collected, concentrated and dried, separated and purified by liquid chromatography to obtain new chlorogenic acid and chlorogenic acid with yields of 0.03-0.05% and 0.03-0.06%, respectively. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107840799-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107840799-A |
| priorityDate | 2014-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID468285633 |
Total number of triples: 22.