| abstract |
The invention relates to selection of the embryo of a test tube baby through sequencing by the single cell genome of a polar body or embryo. The invention discloses a method for carrying out whole genome non-index amplification and genome high-flux sequencing by utilizing a first polar body, a second polar body and the single cell of an embryo in order to realize hereditary disease pre-implantation genetic diagnosis and recurrent abortion pathogenic gene. The method comprises the following steps: 1, obtaining an oocyte and an embryo, and carrying out first polar body, second polar body and single embryo cell separation and genome amplification; 2, establishing a sequencing library, sequencing, and carrying out genome bioinformatics analysis to obtain a gene map, chromosome and copy number information of fragments thereof; 3, determining the chromosome ploidy and chromosome fragment deletion, repetition and gene point mutation information of the polar body and the embryo cell; and 4, selecting normal or appropriate embryo, and transplanting. The single cell genetics analysis method has a wide application range, and experiments prove that the effect is reliable. |