http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104004724-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1074 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-01019 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2012-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104004724-B |
| titleOfInvention | Cyclodextrin glycosyl transferases that a kind of maltodextrin substrate specificity improves and preparation method thereof |
| abstract | The invention discloses cyclodextrin glycosyl transferases of a kind of maltodextrin substrate specificity raising and preparation method thereof, belong to genetic engineering and enzyme engineering field.The present invention is by replacing with serine (Ser) to the tyrosine (Tyr) of 195 of the CGTase of P.macerans strain JFB05 01 (CCTCC NO:M208063), the tyrosine (Tyr) of 260 replaces with arginine (Arg), the glutamine (Gln) of 265 replaces with lysine (Lys), AA 2G yield is made to be respectively increased 23%, 44% and 40%.To above mutant combinatorial mutagenesis, it is thus achieved that double-mutant Y195S/Y260R, Y195S/Q265K and Y260R/Q265K and three Point mutont Y195S/Y260R/Q265K.They utilize maltodextrin to produce AA 2G yield for glycosyl donor and have been respectively increased 57%, 49%, 55% and 59%.These mutants are more conducive to utilize maltodextrin to produce AA 2G for glycosyl donor than wild type CGTase. |
| priorityDate | 2012-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.