http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104004717-B
Outgoing Links
Predicate | Object |
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classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 |
filingDate | 2014-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104004717-B |
titleOfInvention | The universal monoclonal antibody hybridoma cell strain of one strain aspergillus flavus resisting toxin and application thereof |
abstract | One strain aspergillus flavus resisting toxin general purpose single monoclonal hybridomas cell strain and application thereof, belongs to food safety field of immunodetection.Cell strain 1G6 of the present invention, has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, does is deposit number CGMCC? No.7210.Aflatoxin complete antigen and equivalent QuickAntibody immunological adjuvantn tM after mixing, by leg muscle injecting immune BALB/c mouse.First time immunity B1-KLH, second time immunity M1-KLH, the last M1 of using complete antigen (not containing adjuvant) impacts immunity.The splenocyte of immune mouse is merged by PEG method and murine myeloma cell, screens and three subclones through indirect ELISA and indirect competitive ELISA, obtain this strain of hybridoma strain 1G6.The monoclonal antibody of this cell strain secretion, has good avidity and detection sensitivity to aflatoxin B1, B2, G1, G2 and M1, can be used for total aflatoxin content immunodetection in food safety. |
priorityDate | 2014-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.