http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104004681-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-31 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-741 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-00 |
| filingDate | 2014-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104004681-B |
| titleOfInvention | A kind of product gentamicinC2 a engineering bacteria and application thereof |
| abstract | The invention belongs to field of antibiotic pharmacy, relate to a kind of main generation gentamicinC2 a engineering bacteria and application thereof.By analyzing deep red micromonospora gentamycin biosynthetic process, in conjunction with Bioinformatics Prediction, directional transformation gentamycin biosynthetic metabolism stream, improve the accumulation of gentamicinC2 this component of a, be substantially reduced the accumulation of other component C1a, C1, C2 simultaneously.Adopt the method knocked out in gene frame, the epimerase gene in inactivation gentamycin biosynthetic process<i>genB2</i>, including the structure of shuttle plasmid pZB303, pZB303 converts to deep red micromonospora G1008, screens breaking engineering bacterium, fermenting and producing, metabolite detection and component analysis.Main product gentamicinC2 a of the present invention, has filled up and both at home and abroad industrialization cannot produce the blank of gentamicinC2 a so far, utilized this engineering bacteria to produce C2a, and technique is simple, cost is low, has great application prospect in field of antibiotic pharmacy. |
| priorityDate | 2014-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.