http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104004098-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 |
| filingDate | 2014-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104004098-B |
| titleOfInvention | A kind of carrier compositions and application that utilizes Wnt signal activated state in BiFC indicator cells |
| abstract | The invention discloses system and the application of Wnt signal activated state in a kind of BiFC of utilization indicator cells. The invention provides a kind of fusion group, be made up of fusion A and fusion B, described fusion A comprises core effector β-catenin fragment and is positioned at the fluorescin Segment A of its C section; Described fusion B comprises transcription factor TCF and is positioned at the fluorescin fragment B of its N section. Of the present invention experimental results show that, the present invention utilizes complex core effector β-catenin in Wnt signal path and transcription factor TCF being formed according to the strategy of bimolecular fluorescence complementary technology (BiFC) visual, thereby directly show the activated state of Wnt in cell, the present invention is by BiFC system transient transfection cell or be built into stable expression cell line, can be observed the special signal forming in nucleus, this signal can special demonstration and is reflected Wnt signal path activated state and variation thereof in living cells. |
| priorityDate | 2014-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 98.