http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103992991-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-01024 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-25 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-14 |
filingDate | 2013-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103992991-B |
titleOfInvention | A kind of bile salt hydrolase mutant and application thereof |
abstract | The invention discloses a kind of bile salt hydrolase mutant and application thereof, belong to gene engineering technology field.The present invention passes through the homology modeling to the analysis of bile salt hydrolase amino acid alignment and 3-D structure and overlay analysis, determine bile salt hydrolase substrate specificity amino acid mutation site, utilizing site-directed mutagenesis technique is nonpolar amino acid by substrate specificity amino acid mutation, mutant is imported Host Strains, it is thus achieved that mutant strain.The produced bile salt hydrolase of mutant is organized enzyme, and compared with wild type, sweet ammonia is all had a declining tendency by mutant in conjunction with the hydrolysis ability of cholate, and cattle sulphur then has the trend of increase in conjunction with the hydrolysis ability of cholate.The binding mechanism of substrate is laid a good foundation with disclosing it in conjunction with the hydrolysis ability of cholate by this by cattle sulphur for improving BSH. |
priorityDate | 2013-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.