http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103988777-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
filingDate | 2014-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103988777-B |
titleOfInvention | A kind of in-vitro culture method for tender stem segments of the wide yulan of leaflet dwarf form |
abstract | The present invention relates to a kind of in-vitro culture method for tender stem segments of the wide yulan of leaflet dwarf form, belong to field of plant tissue culture technique.Described method is comprised the following steps:1) selection and pretreatment, 2 of inoculation material, are intended), explant sterilization, 3), aseptic strain foundation, 4), Multiple Buds induction and Multiplying culture, 5), strong sprout and culture of rootage, 6), test tube seedling hardening with transplant.The device have the advantages that:The inductivity of Multiple Buds reaches more than 90%, rooting rate after strengthening seedling and rooting culture reaches more than 96%, survival rate is up to more than 96%, reproduction speed is fast, breeding potential is high, virus-free and purification and rejuvenation the advantages of, overcome the defects such as breeding coefficient in the prior art is low, the cycle is long, be subject to seasonal restrictions, can in a short time factorial praluction high quality seedling meeting the need of township afforestation.By taking a stem section as an example, in 1 year can batch production quickly breed seedling strains up to ten thousand, and good quality. |
priorityDate | 2014-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.