http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103983749-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e89c206df73c7928c3fa05f87f1287de |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-12 |
| filingDate | 2014-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_48ab5c094b8599318bf9ea9eee521381 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_36979621cace70854a9b263368494ae4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7958cc87529faddd8b611f4f777d701d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9a283d0fbfe48e8f774902b4efa1211 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f36d837380d164d6a0ead267cdb0f9b |
| publicationDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103983749-B |
| titleOfInvention | Capillary immunity-chromatography rapid detection method for parvalbumin in aquatic products |
| abstract | A disclosed capillary immunity-chromatography rapid detection method for parvalbumin in aquatic products comprises the following steps: 1, preparing a to-be detected sample, specifically mixing fish flesh with Tris-HCl, homogenizing, filtering, heating in a water bath, centrifuging, and colleting a supernatant as a to-be detect sample; and 2, detecting the sample, specifically mixing uniformly a first antibody labeled by colloidal gold with the to-be detected sample to obtain a mixed solution, fetching 5-15 mu L of the mixed solution, injecting into the end of a detection zone of an immunity-chromatography capillary, standing for 4 min, pushing the mixed solution to flow downwards to a quality-control zone of the immunity-chromatography capillary by using a pipettor, also staying for 4 min at the zone, discharging excessive solution out of the capillary, then immersing the immunity-chromatography capillary in a PBST buffer for twitch cleaning, and finally obtaining a detection result through naked-eye qualification. The method is good in detection sensitivity, high in repeatability, short in detection time and good instability, and is applicable to instant rapid detection. |
| priorityDate | 2014-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.